We would like to deplete alveolar macrophages in mice and we cannot find suitable antibodies (F4/80?). We will be using random Swiss mice (non isogenic).
The only way I found is by using clodronate liposomes with yield efficacy varying from paper to another one.
Any recommendations?
Thanks in advance
Clondronate liposomes are the easiest and most efficient way to deplete alveolar macrophages. Most antibodies are not specific and not effective. The dose and the type of application of the liposomes may influence efficacy.
As mentioned by G. Zissel, clodronate-liposomes are the method of choice to deplete alveolar macrophages. The technique of pulmonar liposome delivery is somewhat difficult. Liposomes can be applied by intranasal injection which is technically easy but which does not guarantee high reproducibility because an unknown portion of the applied liposomes can also be swallowed. The more reliable application is the intratracheal way where an incision in the trachea has to be made which is a small surgical intervention. The method is described for example in: The depletion of donor macrophages reduces ischaemia-reperfusion injury after mouse lung transplantation. Tsushima Y, Jang JH, Yamada Y, Schwendener R, Suzuki K, Weder W, Jungraithmayr W. Eur J Cardiothorac Surg. 2014 Apr;45(4):703-9