Does anybody know a fluorescent dye for lysosomes in fix cells? This question is because the only stain I´ve founded is for living cells.
you can use lysotracker red from invitrogen. You have to incubate it with live cells, but it survives fixing in 4% paraformaldehyde (20 min). I can't think of a lysosome specific dye that can be applied after fixing...
Hi Dr. Rice,
Thanks for your comments. I really appreciate it.
regards
Hi Aaron . thank you very much for your help
LAMP-1 or LAMP-2 are also lysosome marker that you can use to staining fixed cells.
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