No title

Hi everybody, Does somebody know how many times can I reused the tips from piettors ??? regards Patricia

07 August 2011 2,760 14 View

Aspects about IVF room

07 August 2011 6,897 1 View

No title

Hi everybody, Does somebody know how many times can I reused the tips from piettors ??? regards Patricia

07 August 2011 1,036 7 View

LIPID PEROXIDATION

HI EVERYBODY, DOES ANYBODY KNOW WHAT CAN I USED TO MEASURE LIPID PEROXIDATION BY GREEN FLUORESCENCE ??? REGARDS PATTY

31 December 2010 6,236 1 View

LIPID PEROXIDATION

HI EVERYBODY, DOES ANYBODY KNOW WHAT CAN I USED TO MEASURE LIPID PEROXIDATION BY GREEN FLUORESCENCE ??? REGARDS PATTY

31 December 2010 5,613 0 View

LIPID PEROXIDATION

HI EVERYBODY, DOES ANYBODY KNOW WHAT CAN I USED TO MEASURE LIPID PEROXIDATION BY GREEN FLUORESCENCE ??? REGARDS PATTY

31 December 2010 9,508 3 View

Lipid Peroxidation

Does anybody know what I can use to measure lipid peroxidation by green fluorescence?

31 December 2010 4,295 2 View

LIPID PEROXIDATION

HI EVERYBODY, DOES ANYBODY KNOW WHAT CAN I USED TO MEASURE LIPID PEROXIDATION BY GREEN FLUORESCENCE ??? REGARDS PATTY

31 December 2010 942 0 View

help

HI. Does somebody know what kind of agar I can use to process embryos to Immunohistochemistry???? I hope somebody know and can help me. Thanks in advance

06 July 2010 2,287 1 View

help

05 June 2010 1,058 5 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

Is there a way to tell based on appearance which protein crystal should diffract the best?

I have had unheard of success with protein crystallography lately from a super successful protein expression and purification batch. I have attained a lately reproducible vast amount of crystals...

09 February 2021 5,155 5 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

How to solve error "Cannot find position restraint file restraint.gro (option -r)." on GROMACS?

Hi, I am about to do energy minimization but the above error occurred, I checked my .mdp file and .top file but could not find the problem. TOP file ; Include forcefield parameters #include...

01 February 2021 780 3 View

Why is Fermented Plant Juice (FPJ) always diluted with water? Could it be diluted with other solvents such as rice water?

I am currently making a research paper with my classmates in Philippine Science High School and we are trying to find references that mixed Fermented Plant Juice with other solvents instead of...

17 January 2021 5,099 4 View

Any stable dye to color acrylamide hydrogel beads?

Hi! We are generating hydrogel beads(50-60um) using microfluidics as single-cell beads. To simply all the experiments (especially the centrifuge), I am looking for a dye. The dye should be stable...

14 January 2021 4,383 8 View

How to phosphorylate specific residues on pymol?

Hi everyone, I am using the pyTMs plug in on Pymol to phosphorylate a particular threonine in my protein. I am having difficulty in selecting only one or two residues - it seems I can only...

05 January 2021 230 3 View