Hi everybody,
Does somebody know how many times can I reused the tips from piettors ???
regards
Patricia
For molecular work...No! but yes you can reuse tips after proper washing and sterilization for general microbiology work. Nothing can be said about number of times, it depends on material of tips or handling.
For molecular works, tips shouldn't be reused, may be for microbiology works, reuse your tips after proper serialization
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Thank you Dr. Chidambaram. But in case tips be reuse how many times are they possible to reuse?
thanks in advance,
Which experiment exactely are you doing with your tips? In any case it cannot really be a problem of how many times but when do you have to change the tips with regards to the experiment you're doing. For instance when I do in situ hybridization on wholemount embryos, once the probe has been hybridized I can reused the same tips everytime to remove the solution from the tube containing the embryo when changing solution till the end of my experiment. However I will always have to use a clean tip when pipetting from a stock solution. Also when adding the same solution to several different tube you can re-use the same tips several time as long as it doesn't touch the neck of your stock solution bottle or the tube you're adding the solution to.
HI Stephanie, actually I´m working on in vitro fertilizacion. Thank you very much for your advice.
Best wishes,
Patricia
I would most definitely used only clean tips for this kind of procedures!
Do sterilization properly.prefer autoclave .and use it till any physical damage seen. There isnt any harm in reusing.
I'm a microbiologist and an environmentalist too...i dont prefer use n throw of plastics. Its cheap but not recyclable.
Washing and autoclaving is best for pipette reusable, but for pcr or other molecular handling always prefer new one, also avoid using tips after transfer any blood sample
For molecular work...No! but yes you can reuse tips after proper washing and sterilization for general microbiology work. Nothing can be said about number of times, it depends on material of tips or handling.
Tips is cheap, but our environment is not so? try to re use your tips for micro works, but dont for molecular works. As everyone stated,
1.Decan your used tips,
2.Wash properly
3..Sterlize in autoclave and re use it.
Follow this RRR,
Reduce, Recycle, Reuse it
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