I want to know time required by SH-SY5Y cell line to differentiate and which media is suitable for its best growth and differentiation.
My group was the first to publish that retinoids in combination with BDNF induced differentiation in the SY5Y neuroblastoma tumor cell line in 1995. I would recommend differentiation with uM (microMolar) not mM (milleMolar) concentrations.
Helloy, there is many protocols about that. People mainly do it with Retinoic acid(RA) or RA + BDNF.
I attached an article that I am kinda following it.
"Briefly, SH-SY5Y cells were cultured with 10% complete medium (minimum
essential medium, DMEM, 10% heat inactivated fetal bovine serum, L-glutamine (2 mM), penicillin (50 U/ml), streptomycin (50 mg/ml)) in a humidified, 5% CO2, 37C incubator. Forty-eight hour after seeding, serum levels of the medium were reduced to 3%with RA (10 mM) for differentiation for seven days prior to treatment."
obtained by the following research paper.
Hi,
We also use RA, but our group just published a paper also using PMA (also called TPA), see the following link to our recent paper right here on Researchgate.
Good luck!
Article FAT1 cadherin acts upstream of Hippo signalling through TAZ ...
My group was the first to publish that retinoids in combination with BDNF induced differentiation in the SY5Y neuroblastoma tumor cell line in 1995. I would recommend differentiation with uM (microMolar) not mM (milleMolar) concentrations.
- Badges
- Science method