Fu Hung Shiu

6 Questions 7 Answers 0 Followers

Questions related from Fu Hung Shiu

Why microplate reader counter does not match with cell counting by bare eyes?

Hello, I am doing a neuroprotective in vitro study on shsy5y cell culture. By using live/dead assay, it will stain live cells as blue, and dead cells as red. I used fluorescence microplate reader...

11 August 2015 8,733 2 View

Has anyone used Ambion® Cells-to-CT™ Kits for M17 neuroblastoma cells?

I am planning to do a real time RT-PCR BDNF gene expression for M17 cells. I am wondering anyone has ever used Cells-to-CT™ Kits for M17 ( SK-N-BE(2) neuroblastoma cell line) Or anyone can...

11 August 2015 5,981 1 View

What plate should I be using for differentiated SH-SY5Y cells?

Hello, I am interested in counting the live and dead differentiated SH-SY5Y cells by using live/dead assay for my neurotoxic experiment. However, the dead SH-SY5Y cells are easier to float or...

16 July 2015 3,780 2 View

Does the PDL has any inverse effect toward Sh-SY5Y cells?

My lab is studying neuroprotective effect towward Sh-SY5Y cells. However, when I seed the Sh-Sy5Y cells in Sigma 96 well plate coated with PDL (cls3842), 3 days later, all Sh-SY5Y cells clumped...

15 June 2015 3,736 4 View

Should I use LIVE/DEAD® Viability or MTT assay to determine the percentage of surviving cells in 6 ODHA?

Hello, My lab is testing the % surviving cell rate(neuroblastoma SH SY5Y) under 6-ODHA. By doing literature search, we realized that people tend to use MTT assay for determine % surviving cell...

09 February 2015 8,456 5 View

Is there any way to measure real time bdnf in epileptic rats?

Hello, Our group is interested to see the bdnf level in real time in epileptic rats since there are studies showing bdnf can increase seizure level. We are interested in detecting bdnf level for...

07 November 2014 2,780 3 View