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Questions related from Fu Hung Shiu
Hello, I am doing a neuroprotective in vitro study on shsy5y cell culture. By using live/dead assay, it will stain live cells as blue, and dead cells as red. I used fluorescence microplate reader...
11 August 2015 8,731 2 View
I am planning to do a real time RT-PCR BDNF gene expression for M17 cells. I am wondering anyone has ever used Cells-to-CT™ Kits for M17 ( SK-N-BE(2) neuroblastoma cell line) Or anyone can...
11 August 2015 5,977 1 View
Hello, I am interested in counting the live and dead differentiated SH-SY5Y cells by using live/dead assay for my neurotoxic experiment. However, the dead SH-SY5Y cells are easier to float or...
16 July 2015 3,776 2 View
My lab is studying neuroprotective effect towward Sh-SY5Y cells. However, when I seed the Sh-Sy5Y cells in Sigma 96 well plate coated with PDL (cls3842), 3 days later, all Sh-SY5Y cells clumped...
15 June 2015 3,729 4 View
Hello, My lab is testing the % surviving cell rate(neuroblastoma SH SY5Y) under 6-ODHA. By doing literature search, we realized that people tend to use MTT assay for determine % surviving cell...
09 February 2015 8,450 5 View
Hello, Our group is interested to see the bdnf level in real time in epileptic rats since there are studies showing bdnf can increase seizure level. We are interested in detecting bdnf level for...
07 November 2014 2,774 3 View