Why PCR products of Candida spp. using universal primer differ in length depends on species however apply same primer and PCR program?

I am thinking about conservative site PCR product. When PCR products were amplified by universal primer ITS 1and ITS4 region of rDNA, the products length differ among Candida species. Although the...

04 May 2019 1,444 4 View

Three-dimensional Gel Electrophoresis?

Does anybody have information regarding using 3D electrophoresis in the studying gene expression? Does this method could analyse the expression of all genes in the organism or it target specific...

27 November 2018 8,071 3 View

Does anyone could help with NCBI website?

Hi I have new sequence and I want to download in the NCBI website and get reference number. Does anybody can share the steps of this process? Regards Ayat

06 July 2018 7,853 0 View

Does anybody have an idea regarding minus value in statistic analysis?

18 May 2017 5,383 10 View

Does anybody have an idea regarding minus value in statistic analysis?

04 May 2017 2,444 11 View

Does anybody used the formula below?

I want to count killing percentage by using MTT assay. I incubated spores with macrophage in time course and used MTT dye for overnight, dye concentration 0.1 mg/ml. My question is that I want to...

07 August 2016 7,675 2 View

How can I select suitable label and dye in flow cytometer?

Dear all, I am trying to count alive spore from dead one after incubation with macrophage by using flow cytometer, Does anybody has an idea, how can I select suitable label to my spores and...

06 July 2016 9,992 5 View

Does anybody comfirmed the TOXA:GFP transformants with southern blot?

I transformed my strain with TOXA:GFP and trying to confirm the insertion by using southern blot technique. I can see the fluorescent under the microscope but it does not work with ELISA or...

27 April 2016 2,870 2 View

PCB1530 sequence

Does anyone has PCB1530 plasmid sequence or at least has the multiple cloning site? Thank you

31 December 2015 3,167 4 View

How long is needed to repair the DNA damage by photoreactivity?

I read about DNA repair after exposure to UV light, photoreactivity is one of the mechanisms of repair. This mechanism is performed in the presence of light. I am wondering, if somebody exposure...

10 November 2015 9,254 2 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View