Why PCR products of Candida spp. using universal primer differ in length depends on species however apply same primer and PCR program?

I am thinking about conservative site PCR product. When PCR products were amplified by universal primer ITS 1and ITS4 region of rDNA, the products length differ among Candida species. Although the...

04 May 2019 1,444 4 View

Three-dimensional Gel Electrophoresis?

Does anybody have information regarding using 3D electrophoresis in the studying gene expression? Does this method could analyse the expression of all genes in the organism or it target specific...

27 November 2018 8,071 3 View

Does anybody have an idea regarding minus value in statistic analysis?

18 May 2017 5,383 10 View

Does anybody have an idea regarding minus value in statistic analysis?

04 May 2017 2,444 11 View

Does anybody used the formula below?

I want to count killing percentage by using MTT assay. I incubated spores with macrophage in time course and used MTT dye for overnight, dye concentration 0.1 mg/ml. My question is that I want to...

07 August 2016 7,675 2 View

How can I select suitable label and dye in flow cytometer?

Dear all, I am trying to count alive spore from dead one after incubation with macrophage by using flow cytometer, Does anybody has an idea, how can I select suitable label to my spores and...

06 July 2016 9,992 5 View

Does anybody comfirmed the TOXA:GFP transformants with southern blot?

I transformed my strain with TOXA:GFP and trying to confirm the insertion by using southern blot technique. I can see the fluorescent under the microscope but it does not work with ELISA or...

27 April 2016 2,870 2 View

Does anybody comfirmed the TOXA:GFP transformants with southern blot?

I transformed my strain with TOXA:GFP and trying to confirm the insertion by using southern blot technique. I can see the fluorescent under the microscope but it does not work with ELISA or...

03 April 2016 8,458 2 View

PCB1530 sequence

Does anyone has PCB1530 plasmid sequence or at least has the multiple cloning site? Thank you

31 December 2015 3,167 4 View

How long is needed to repair the DNA damage by photoreactivity?

I read about DNA repair after exposure to UV light, photoreactivity is one of the mechanisms of repair. This mechanism is performed in the presence of light. I am wondering, if somebody exposure...

10 November 2015 9,254 2 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Any suggestions for a website that allows you to create questionnaires and gather unlimited data (Totally free)?

I have found only four of them; www.surveyplanet.com www.online-anket.gen.tr www.surveey.com and of course google forms. Expecting to see your suggestions if there is any. Thank you

28 February 2021 3,237 3 View

Do I need to ask permission to use pictures for my questionnaire?

I would like to use some photo from a government website for my questionnaire. But, different photo is from the different owner. So, should I just get permission from that government body or still...

23 February 2021 1,631 3 View

How can I get the coding gene sequences of Hela and U2OS cell lines? Is mRNA sequence of Hsp90 alpha tissue specific??

I am looking for hsp90 mRNA sequence in Hela and U2OS cell lines, I have tried through ncbi but always the output is hsp90 in homo sapiens not in the specific cell line. Kindly, could any one...

22 February 2021 1,853 3 View

How can I retrieve FASTA sequence from gene ID or UID of a Gene ? I have GeneBank IDs ?

I treid to use Entrez Batch, but it redirects me to NCBI site. Is there any script of Linux or Python through which I can download Fasta sequence of list of gene ID ?

21 February 2021 7,783 12 View

How do you interpret multiple meta-regression in STATA?

Please could someone recommend a resource that explains performing meta-regression with multiple moderators. I have read the STATA META ANALYSIS REFERENCE MANUAL, but this only gives examples of...

17 February 2021 7,376 1 View

Can anyone pls provide me information regarding the libraries where from i can get the absorption data of different materials used in solar cells??

I want to create .abs file ( absorption) for simulating solar cell in SCAPS. Can anyone pls provide me information regarding the libraries (websites) where from i can get the absorption data of...

10 February 2021 6,034 7 View

Glucose 6 phosphate dehydrogenase deficiency & NSAIDs - is this combination harmless ?

G6PD deficiency is quite common in our anesthesia practice in the middle east. Intraop/postop analgesia in them is an issue here. Many websites/books give contrasting advise with former advocating...

10 February 2021 4,817 3 View

Up to what extent website personalization can help in creating urge to buy impulsively?

Up to what extent website personalization can help in creating urge to buy impulsively? The digital transformation is making most of the organizations to go online and start doing business through...

09 February 2021 4,430 6 View