I want to count killing percentage by using MTT assay. I incubated spores with macrophage in time course and used MTT dye for overnight, dye concentration 0.1 mg/ml.
My question is that I want to use the formula below but the problem is when I used, I got negative result. Does anybody used before?
Percent killing = 1 - (A 570 of fungi incubated with white blood cells/A570 of. fungi incubated without white blood cells) x 100
Hi Ayat: Theoretically the fungus+white blood should give you higher killing of fungus spore but less live spores and fungi incubated without white blood higher live fungus spore and less killed spore.
so you should count the live spores for bothe of the data and use it in the formal for both data, you will say we have ?? of live spore in (fungi incubated with white blood) it should be low number , and ?? live spore in (fungi incubated without white blood) it should be higher number of live spore.
I hope this clarifies and helpful.
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