Staining methods for skeletal muscle fiber typing?

does anyone know the best method to differentiate and quantify rapid fatiguing and fatigue resistant muscle fibers? the old method form the 1980's uses ATP staining which is quite labor intense?

21 February 2021 6,483 3 View

Does anyone have a recommendation for a book about Raman related to carbon besides Dresselhaus?

I need more than specifically graphene-related raman. I would like a book more related to changes in various carbon-chains. Thank you.

26 August 2020 5,101 3 View

Does anybody have any knowledge of "dry" microbiological decontamination methods for frozen fruit?

I am looking for data on the implementation of a microbiological reduction step for frozen fruit pieces. I have information on cold plasma/UV but am unable to locate much data on gaseous methods...

24 November 2019 6,131 8 View

Is there an analysis method like Grade of Membership Modeling but for continuous (longitudinal) data?

I've been reading up on grade of membership models and they may be promising for some analysis I would like to do, but seem to be mostly for categorical data. I've heard of mixed and partial...

19 November 2019 6,577 2 View

How do you see earth's periodic revolutions through the universe (resulting in force majeures) influencing patterns of climate and evolution?

"Fundamentals of Hydrology" Book Review By: Maryellen Elizabeth Hart Geology 110 Summer 2018 Bz 220 Evolution June 24, 2018 and June 12, 2019 Quick summary: Edited version of my book review of...

10 June 2019 5,363 3 View

Does anyone have experience with the synthesis of palmitate-BSA and the subsequent uptake by the HuH7 cell line?

12 February 2017 4,538 3 View

PX458 transfection in HEK293 cells?

17 October 2016 7,023 6 View

How can I purify a recombinant H 10 tagged N protein from a co-contaminant post IMAC SEC?

09 May 2016 6,765 4 View

Is there a lab that has the experience and would like to collaborate on a neurokinin receptor binding autoradiography study?

It would require Iodinated ligand and microscopic quality resolution of the receptor labeling for correlation of anatomical sites.

12 January 2016 3,628 2 View

Do you have a rotocol to make osteologic discs?

Does anyone have a protocol to make osteologic discs? E.g. hydroxyapitate discs. BD bioscience no longer produce them and the one protocol I have takes 3 weeks.

19 March 2015 5,358 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View