Lesley Ruth Hart

4 Questions 5 Answers 0 Followers

Questions related from Lesley Ruth Hart

Does an immortalized gastric epithelial cell line exist?

Do these exist!? Does anyone have any experience with gastric epithelial cell lines?

19 May 2015 10,195 1 View

Do you have a rotocol to make osteologic discs?

Does anyone have a protocol to make osteologic discs? E.g. hydroxyapitate discs. BD bioscience no longer produce them and the one protocol I have takes 3 weeks.

20 March 2015 5,566 3 View

Which are the best markers for RAW264.7 differentiation into osteoclasts-like cells by western?

I'm trying to differentiate RAW 264.7 cells into osteoclast-like cells using RANKL. By eye I can see that multi-nucleated osteoclasts are forming by day 3, but when I blot for proteins such as...

19 September 2014 4,355 3 View

What is the best Lysis solution for making protein extracts from differentiated RAW264.7 cells (osteoclasts)

I'm simply trying to make protein extracts of my differentiated RAW cells (osteoclast-like) for a western. I have tried using 1% triton and also 0.% NP-40 lysis buffers but the Bradford readings...

07 July 2014 720 3 View