Hello, I am Pieter and I am currently doing research that involves determining the total terpenoid content in a sample.
The test I want to do is based on the article from Ghorai et al. (2012) that estimated the total terpenoids concentration in plant tissues using linalool as standard reagent. In this test a reddish brown precipation will be formed after a sample has been exposed to chloroform and subsequently sulphuric acid.
Can anybody explain how this reddish brown precipitation is formed?
Thank you.