The Role of Town Planners in CAV Use?

Colleagues and I are about to begin a project investigating the role of town planners and local authorities in establishing the introduction of connected and autonomous vehicles (CAVs). What...

21 February 2021 2,247 3 View

Does anybody have any knowledge of "dry" microbiological decontamination methods for frozen fruit?

I am looking for data on the implementation of a microbiological reduction step for frozen fruit pieces. I have information on cold plasma/UV but am unable to locate much data on gaseous methods...

24 November 2019 6,131 8 View

How can I purify a recombinant H 10 tagged N protein from a co-contaminant post IMAC SEC?

09 May 2016 6,765 4 View

Is there a lab that has the experience and would like to collaborate on a neurokinin receptor binding autoradiography study?

It would require Iodinated ligand and microscopic quality resolution of the receptor labeling for correlation of anatomical sites.

12 January 2016 3,628 2 View

Does an immortalized gastric epithelial cell line exist?

18 May 2015 10,013 1 View

Do you have a rotocol to make osteologic discs?

Does anyone have a protocol to make osteologic discs? E.g. hydroxyapitate discs. BD bioscience no longer produce them and the one protocol I have takes 3 weeks.

19 March 2015 5,358 3 View

What is the difference between the 'sociocognitive account' of discourse and cognition (van Dijk, 2006), and discursive Psychology's account?

The SC account of discourse argues against a direct contextual account in the analysis of discourse, proposing that such an account is limiting. Van Dijk argues that it is important - essential -...

17 November 2013 6,012 3 View

Can anyone please suggest a good protocol or assay to detect and quantify mature microRNAs (miRNAs) using real-time PCR instruments

17 February 2012 5,087 22 View

Functional skills assessment and AI

Does anyone know of any organisation that is using AI in functional skills (English and Maths) assessment (adults)? Extensive searches reveal no products or services on the market which leverage...

01 January 1970 1,986 6 View

Glass fiber content in a gypsum board ?

Any idea on how to get the volumic ratio of glass fiber in a gypsum board ? Thank you for your help !

01 March 2021 2,501 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

How can we limit artifacts caused by rotation in fiber photometry?

We are trying to run fiber photometry experiments for long time scales, but we get these sinusoidal artifacts that come up. We think they may be caused by twisting of the fiber optic subject...

25 February 2021 568 1 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View