How can I prevent bubbles from forming under a coverslip?

My colleague and I are having some trouble mounting 70 micrometer mouse brain slices. When we initially position the coverslip over the mounted slices, there are no bubbles but when we come back...

10 February 2021 3,580 1 View

Can anyone suggest why my PCR results gave big differences in the control group?

I did RT-PCR using SYBR Green and used 2 ul of cDNA per sample to investigate the effect of LPS in primary osteoclast culture. I loaded the same amount of RNA to make cDNA (550ng/sample). However...

17 September 2020 9,193 3 View

Hi, can anyone clear up about the molarity concept?

I'm required to prepare 2mM of LiCl for my DNA extraction method, which I calculated to be 0.0212 g of LiCl in 250 ml of water. My supervisor told me to use any volume as long as it is 2 mM....

14 February 2020 9,808 3 View

Is there an analysis method like Grade of Membership Modeling but for continuous (longitudinal) data?

I've been reading up on grade of membership models and they may be promising for some analysis I would like to do, but seem to be mostly for categorical data. I've heard of mixed and partial...

19 November 2019 6,577 2 View

How do you see earth's periodic revolutions through the universe (resulting in force majeures) influencing patterns of climate and evolution?

"Fundamentals of Hydrology" Book Review By: Maryellen Elizabeth Hart Geology 110 Summer 2018 Bz 220 Evolution June 24, 2018 and June 12, 2019 Quick summary: Edited version of my book review of...

10 June 2019 5,363 3 View

I would like to find out why QGIS to Web is not used in academic research of Web GIS?

I would like to find out why QGIS to Web is not used in academic research of Web GIS?

15 May 2018 9,351 3 View

About online business acceptance journals?

Hi, Can someone please recommend what ISI or Scopus indexed journals that I can submit my manuscript ? my study is about what is people perception/acceptance of online business in asia? whether...

31 January 2018 7,181 7 View

Does anyone have experience with the synthesis of palmitate-BSA and the subsequent uptake by the HuH7 cell line?

12 February 2017 4,538 3 View

PX458 transfection in HEK293 cells?

17 October 2016 7,023 6 View

How can we speed up the time of publishing students work, without turn a frustration for their career? Is it getting worse?

Some data suggest that wait times have increased within certain subsets of journals, such as popular open-access ones and some of the most sought-after titles. At Nature, the median review time...

10 February 2016 7,715 9 View

Could you recommend any books about Number Theory?

Beginner level books. High level books. Analytical Number Theory. Algebraic Number Theory. Combinatorical Number Theory. Books about partitions. Books about primes.

03 March 2021 7,174 5 View

What kind of intervention will be relevant to increase motivation among young sport trainees?

Any suggestion on what book to read is also welcome.

03 March 2021 1,646 4 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

How to quantify carbon deposition on CuZnAl catalyst?

Dear All, I want to quantify carbon deposition on the surface of the CuZnAl catalyst. I have tried thermogravimetric analysis, but I've got weight gain as the active Cu- get oxidised. Does anyone...

01 March 2021 1,907 3 View

Why Trivalent chromium start precipitating above PH 5 ? Moreover discuss difference between adsorption of chromium and precipitation of chromium ?

I am a student of MS Environmental Engineering and started my research phase. I am working on metal removal ( chromium iii ) by using activated carbon prepared from the agricultural feedstock. So...

01 March 2021 5,475 2 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View