01 January 2015 4 6K Report

Hello,

I've read a lot online that we are supposed to check that the pH of Towan Buffer is ~8.3 however I couldn't find whether that should be the pH of the 1x buffer after addition of methanol or before addition of methanol.

I usually add 20% methanol to my 1x of 25 mM Tris base and 192 mM Glycine. This is for a wet transfer using the Tank.

Thanks!

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