Sarah Sh

6 Questions 7 Answers 0 Followers

Questions related from Sarah Sh

Can someone provide help in Polsyome profiling-- making the buffer?

Hello,I'm trying to make the hypotonic buffer for polysome profiling and I was wondering if anyone can help me out with the reasoning behind why there are various recipies.I'm stuck using HeLa...

03 March 2015 1,922 2 View

How do I solve my problem while transfecting siRNA into RH41 and RD cell lines?

I just started working with RD and RH41 rhabdosarcoma cell lines. They seem to be difficult to deal with so far. They grow very slowly.I tried to transfect both cell lines with different...

02 February 2015 414 7 View

Do you test the pH of Towan Buffer when it is 10x or after you diluted it to 1x ?

Hello,I've read a lot online that we are supposed to check that the pH of Towan Buffer is ~8.3 however I couldn't find whether that should be the pH of the 1x buffer after addition of methanol or...

01 January 2015 6,165 4 View

Is YOYO-1 dye stable at 42°C?

I'm trying to study survival of cells under heatshock stress and I want to use YOYO-1 to look at the amount of cells dying. I'm finding that YOYO-1 is not picking up dying cells as properly as it...

12 December 2014 1,964 2 View

What is the best way to store a PVDF blot after transferring?

I would like to store my blot after transferring, i'm just not sure how to do that. I have once tried to leave my blot in PBST over few days and I found that the signal I got after probing wasn't...

11 November 2014 5,086 2 View

I have having issues with my Blots. When I use Ponceau Stain, I see bands but when I probe with antibodies, I cannot detect anything. Any suggestions?

I usually transfer my gel at constant 300 mA for an hour and a half, using a 10% Tris-glycine gel. I use 15% Methanol. So after I transferred and blocked my blot, I incubated the blot with two...

07 July 2014 9,506 5 View