Hello,

I'm trying to make the hypotonic buffer for polysome profiling and I was wondering if anyone can help me out with the reasoning behind why there are various recipies.

I'm stuck using HeLa cells and i have been told that they are terrible to use for polsyome profiling but like i said i'm stuck with it. 

Are there certain things that I can add to get better RNA lysis? or perhaps something I am not adding that does not allow me to get good RNA amount from HeLa? or are they really just crappy and do not yield a lot of RNA?

The recipe i'm using is 

300 mM NaCl

15 mM MgCl2

15 mM Tris-HCL pH 7.4

Thanks