In flow cytometry, how can separate Synechococcus-PC rich from Prochlorococcus using FACSCALIBUR cytometer?

10 September 2015 10,000 2 View

Why do I obtain two p53 bands with ab26 antibody from abcam?

I am running a series of characterization experiments in different cell lines. When using the p53 [PAb 240] antibody from abcam (ab26) I obtain two bands around 50KDa. Surprisingly some lines have...

27 January 2015 8,119 4 View

Why do I obtain two p53 bands with ab26 antibody from abcam?

I am running a series of characterization experiments in different cell lines. When using the p53 [PAb 240] antibody from abcam (ab26) I obtain two bands around 50KDa. Surprisingly some lines have...

31 December 2014 8,053 5 View

Does anyone know the p53 status (WT, mutated) in liposarcoma cell lines 402-91 and 1765-92?

I am working with two different liposarcoma cell lines who have a different behaviour when treated with several drugs, I am trying to understand if the p53 status has something to do with that but...

09 October 2014 1,740 5 View

Do you know an efficient method to induce apoptosis in sarcoma cell lines?

We want to quantify death response in Ewing's sarcoma cell lines using flow cytometry but we lack a bona fide apoptosis inductor for those cells (they don't even die in serum deprivation).

05 November 2013 1,534 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How to handle studies which report perfect effect sizes in a meta-analysis using Pearson's r?

I'm in the process of updating a meta-analysis and in one of the new studies, effect sizes are reported as Tau-U. Two of the effect sizes are reported as Tau-U = 1.00. I am using Andy Field's...

24 February 2021 2,265 3 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

How to calculate time-averaged fields from Ansys CFX solver?

I would appreciate any comments on the following problem. I need to calculate mean fields (e.g. pressure, velocity and vorticity), for which I activate Trn Stats tab in CFX-Pre. However, what I...

21 February 2021 8,548 7 View

Which software is best to analyse multi-phase fluid flows ?

Hello everyone, hope all is good. I want to analyse multi-phase fluid flow through pipe, which software is best to do so ?

21 February 2021 4,230 8 View

The PC12 cell line characteristics?

Hello dear scientists, Does the undifferentiated PC12 cell line in terms of chromosome characteristics, proliferation, and drug reactions have the characteristics of cancer cells? Where is it...

21 February 2021 8,945 3 View

Can anyone help with an Accurate Diffuser Expansion Loss formula for rectangular shaped diffusers in turbulent flow regime?

I have tried Idelchik's Handbook of Hydraulic Resistance 4th Edition (2007 page 300) formulas and across some range the formulas are reported valid for, the law of conservation of energy is...

20 February 2021 2,504 1 View