Ayman Jafar Oweida

5 Questions 11 Answers 0 Followers

Questions related from Ayman Jafar Oweida

What's the minimum amount of loading buffer and reducing agent that one can use for Western Blot?

I have minimal protein concentration and would like to add the lowest possible amount of loading buffer and reducing agent. We usually use 10x reducing agent and 4x loading buffer. Therefore, if...

05 May 2016 1,807 5 View

Can cells undergoing mitotic catastrophe express senescence-associated beta galactosidase activity?

Although, senescence and mitotic catastrophe are 2 independent processes, I have observed cells with enlarged appearance, highly polyunucleated and with micro-nuceli that stain positive for...

06 June 2015 3,114 4 View

Is anyone doing immunohistochemistry on rat lungs?

Any advice on excising the lungs of rats for paraffin embedding and sectioning would be greatly appreciated.

02 February 2014 7,308 3 View

Why do some cancer cells become more invasive after being treated with high doses of ionizing radiation?

Numerous papers have reported increased invasion of cancer cells after treatment with high doses of ionizing radiation. I have seen this in my work with lung cancer cells as well. Is there a...

09 September 2013 8,861 4 View

Can anyone with experience on implantation of A549 lung adenocarcinoma cells comment on their metastatic potential in animal models?

Insight into an orthotopic animal model would be most relevant.

07 July 2013 8,187 2 View