Already acquired my samples and notice that Synechococcus-PC rich is overlapping with Prochlorococcus on the chart FL4, red vs FL2, orange. What should I do to discriminate each population? Or that software should I use?
Gregory Veltri
If you have single positive controls of these species, you can easily compensate it. If you tried that and the comp is very high, take it to Flowjo.
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Marilyne Guillamin
I use a Gallios cytometer and Kaluza sotware (Beckman Coulter) to do the same analysis. I can identify some populations in FL3/FL4.
You can see the file linked.
Some publications ask of this analyse. Must take into account the changes in fluorescence
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