Can anyone help with murine mesothelioma immunolabeling?

I would like to characterize murine peritoneal mesothelioma using immunolabeling on paraffin-embedded tumor. Can someone indicate me references of primary antibodies that worked, such as antibody...

11 December 2013 9,152 1 View

Can anyone help with murine mesothelioma immunolabeling?

I would like to characterize murine peritoneal mesothelioma using immunolabeling on paraffin-embedded tumor. Can someone indicate me references of primary antibodies that worked, such as antibody...

01 December 2013 4,011 1 View

Can anyone help me with my dot blot analysis?

Performing dot blot with cellular extract and western blot anti-GAPDH, we have obtained irregular labeling of our dots with white labeling in the center. How to explain this labeling? 2.5 ul of...

12 November 2012 7,234 12 View

Can anyone help me with my dot blot analysis?

Performing dot blot with cellular extract and western blot anti-GAPDH, we have obtained irregular labeling of our dots with white labeling in the center. How to explain this labeling? 2.5 ul of...

10 November 2012 7,821 13 View

IL-1 beta antibodies used in western blot

I would like to detect secreted IL-1 beta from human cell supernatant using Western blotting. Has someone tested antibodies directed against IL-1 beta working in WB? Can you recommend references...

24 April 2012 1,992 2 View

IL-1 beta antibodies used in western blot

I would like to detect secreted IL-1 beta from human cell supernatant using Western blotting. Has someone tested antibodies directed against IL-1 beta working in WB? Can you recommend references...

03 April 2012 8,539 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View