Can anyone help with murine mesothelioma immunolabeling?

Véronique Serre-Beinier @Veronique-Serre-Beinier

02 December 2013 1 4K Report

I would like to characterize murine peritoneal mesothelioma using immunolabeling on paraffin-embedded tumor. Can someone indicate me references of primary antibodies that worked, such as antibody directed against calretinin, pankeratin, mesothelin, vimentin, WT-1, ....?

Emanuela Felley-Bosco

Hi Véronique,

MPM cell lines grown in serum have a tendency to loose the nice MPM markers you have observed in the primary tumors. Vimentin should work based on literature.

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