IL-1 beta antibodies used in western blot

More Véronique Serre-Beinier's questions See All

Why do we only study the DC gain in VLC channel modeling?

In all the papers and books that I read, only the DC channel gain of the VLC system is studied without any further justification. How can we know for sure that the channel doesn't affect the...

13 February 2020 6,718 3 View

Do you have information on tumorigenicity in mesothelioma cell lines?

I need to follow development of human mesothelioma cells into athymic nude mice after mesothelioma cell injection sub-cutaneously or intra-pleuraly. In order to evaluate the number of mice to use...

04 May 2014 1,758 1 View

What is the best algorithm to identify miRNA sites in the 3'UTR of a mRNA?

I tried TargetScan, microRNA.org, PicTar and Targetspy and I got different results. Also, when you present the results, do you mention only the conserved miRNAs or the poorly conserved as well?...

20 March 2014 3,211 3 View

Can anyone help with murine mesothelioma immunolabeling?

I would like to characterize murine peritoneal mesothelioma using immunolabeling on paraffin-embedded tumor. Can someone indicate me references of primary antibodies that worked, such as antibody...

11 December 2013 9,152 1 View

Can anyone help with murine mesothelioma immunolabeling?

I would like to characterize murine peritoneal mesothelioma using immunolabeling on paraffin-embedded tumor. Can someone indicate me references of primary antibodies that worked, such as antibody...

01 December 2013 4,011 1 View

How do I determine the molecular mass of smectite?

I have analysed clay particlesn with XRD and a peak associated with smectite was seen. I want to work out how much smectite could be in the clay by using XRF data I also obtained.

13 October 2013 2,416 2 View

Can anyone help me with my dot blot analysis?

Performing dot blot with cellular extract and western blot anti-GAPDH, we have obtained irregular labeling of our dots with white labeling in the center. How to explain this labeling? 2.5 ul of...

12 November 2012 7,234 12 View

Can anyone help me with my dot blot analysis?

Performing dot blot with cellular extract and western blot anti-GAPDH, we have obtained irregular labeling of our dots with white labeling in the center. How to explain this labeling? 2.5 ul of...

10 November 2012 7,821 13 View

IL-1 beta antibodies used in western blot

I would like to detect secreted IL-1 beta from human cell supernatant using Western blotting. Has someone tested antibodies directed against IL-1 beta working in WB? Can you recommend references...

03 April 2012 8,539 2 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

What is the best way to manage Western blots?

Increasingly, more articles are being retracted because of duplicate or otherwise dubious Western blots. Consequently, journals are asking for original Western blots and authentication of...

23 February 2021 8,609 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

Can anyone help me how to get the similar bands in Actin?

I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...

22 February 2021 6,937 3 View

I see 2 random bands in my ERK/pERK blot? Has anyone else seen this?

I ran a western blot using 12% gel and probing for ERK/pERK (cell signaling antibodies). The green is total ERK, the Red is supposed to be phospho-ERK. I see 2 bands below the total ERK that...

22 February 2021 1,777 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Unable to detect a truncated protein variant, but I can observe a truncated cDNA from Sanger?

Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...

17 February 2021 1,130 6 View

(western blot) can methanol damage my antibody signal?

I accidentally rinsed with methanol after adding my primary antibody to my membrane. I noticed after ~10 seconds and immediately replaced it with PBST (3x15 min washes). Will my antibody signal be...

12 February 2021 5,824 3 View

Preeti Aahir

What do you mean by human cell supernant? is ir primary cell line or carcinoma cell lines? It does work very well at higher dilution. and there are kits avialable for that too. This paper shudl help you. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1573068/

Véronique Serre-Beinier

These are supernatants from human primary cells (pancreatic cells). Thanks a lot for the reference.