I do an overexpression of the estrogen receptors in the H9C2 cell line and then treat the cells with estradiol and then measure luciferase assay. But there is no response in luciferase activity. Does anyone have an experience with that?
Thank you for the answer. I checked receptors expression, and I have very it good. I am interested in this particular cell line. I checked different E2 and different concentration and time points. The problem is luciferase by itself. It seems like promoter is not active but I dont know how to confirm that.
May be you need to check your experimental procedure including the time of exposure to E2 and Luciferase induction measurement. You can compare your procedure with the procedures used for other similar cell lines.
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