Hi, I am using a commercial line of NSCs from X Cell Science. I would like to know if anyone has stained it for PAX6 marker? If so, was it positive? Having said that, are NSCs supposed to express PAX6? What if they don't?
NSCs do express PAX6 and its level is essential for determining the self-renewal, multipotency and neurogenesis/differentiation state of the given population.
You may have a look at those two papers for more details: PMID: 19521500 & PMID: 27191603 .