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Questions related from Yojet Sharma
While calculating for K+ where are the cursors supposed to kept at: at the stable traces or near the rise side?
05 May 2024 9,463 1 View
As I approach my recording electrode in the bath, the response drifts from 0 to all the down to -2000. I tried offset option and chlorided the wire (recording as well as one in the bath) with...
09 February 2022 1,667 2 View
There are reports outlining generation of hippocampal/DG specific neurons. But none about EC specific. So is it possible?
03 May 2020 7,751 0 View
Hi, I am using a commercial line of NSCs from X Cell Science. I would like to know if anyone has stained it for PAX6 marker? If so, was it positive? Having said that, are NSCs supposed to express...
31 July 2019 4,245 2 View
I wish to look at gliosis in a dish, basically the neurons start becoming apoptotic and glial cells come to the rescue. But if I have a patient neurons, how do I mimic this event? Any idea what...
09 June 2019 5,981 9 View
I have TTX citrate ordered from Hello Bio. On using it at 1uM as my working concentration, it didn't show any effect. How do I test whether the toxin is actually working or not?
17 February 2018 1,041 4 View
Do they acquire neuron-like morphologies in your cultures as well? What signalling mechanisms are taking place when they're seeded at lower cell density?
03 February 2018 4,996 2 View
Does one add the high-concentration of KCL (~40-50mM) solution directly into the dish as its being imaged? Does the calcium-dye loading needed to be done in the above solution as well?
03 October 2017 8,643 2 View
Are any set of definite protocols that describe the protocol for single-wavelength calcium indicators, where they determine Fmin using EGTA/BAPTA? I wish to avoid in using MnCl2 as it gives...
24 February 2017 3,134 0 View
Just as we calibrate parameters for imaging in different situations (such as checking if a cell sample has been illuminated properly or not, simulating a code for mean square displacement and...
05 February 2017 1,693 2 View
How does the chemistry of calcium dyes work out? What happens during live-cell imaging if the the affinity of one dye to bind to calcium is higher than another?
03 February 2017 8,500 7 View
