Onur Can Begentas

4 Questions 19 Answers 0 Followers

Questions related from Onur Can Begentas

Can KSR levels be reduced in iPSC culture (feeder-dependent)?

I've been generating and culturing multiple different human iPSCs lines on mito-MEF feeders using standard hiPSC media composed of Advanced DMEM/F12 (Gibco) with 20% KSR, pen/strep, glutamax,...

19 November 2021 6,187 0 View

How to culture PBMCs?

Hi there! I've decided to work with reagents from stemcell, namely SFEM II & erythroid expainsion supplement. The purpose is to expand erythroblasts from freshly isolated PBMCs for somatic cell...

02 January 2020 1,046 2 View

Can RPMI 1640 be substituted for StemSpan, StemPro-34 or QBSF-60?

I wanna selectively expand erythroblasts from freshly isolated PBMC for reprogramming into human iPSCs. Would I be able to substitute those above media with RPMI 1640 with cytokines and growth...

18 September 2019 5,764 1 View

How to delete a short sequence with CRISPR/Cas9 on mESCs?

Hello all, I am planning to delete a consequtive 9 nt. sequence (3 codons) from mouse embryonic stem cell genome. I thought CRISPR/Cas9 mediated knock-in would do it. The question is, I am not...

17 July 2019 4,778 8 View