The photo attached were the fibroblast after 48 hours incubation, all the cells could not be "observed" after 48 hours instead of tiny particles was "shaking" in the plates. May I ask that all those shaking particles were mycoplasma or it was due to the serum, Fetal Calf Serum precipitation(heat inactivated)?
yes it may contain mycoplasma if the mother of checks is infected by one of Avian mycoplasma also fetal calf serum could have Mycoplasma arginini that contamin the tissue culture as well but you could not be sure until isolate mycoplasma organism or DNA by pcr .
I completely agree that it sounds like mycoplasma. Of course, contaminations can also result during isolation of the fibroblasts. You can get specific pathogen free (SPAFAS; spf) eggs (e.g. from Charles River).
It seems cell culture is contaminated by mycoplasma. Probable source of most mycoplasma contaminants in primary cell culture is the original tissue used. I agree contaminations could have resulted during isolation of fibroblasts from embryos. Lab equipment, media, or reagents that have been contaminated by previous use should also be considered as a possible source of contamination.
- Badges
- Science method