we know that EMT is critical step for metastasis ,then these metastatic cell require MET in establishment and stabilization of distant metastases. but what about cell lines taken from metastatic site , will they undergo EMT when inject intravenously and thus require MET in order to colonize metastatic site.?
yes they wou.d. EMT is genrally followed by MET for formation of secondary tumors. no matter how metastatic cells are in order to colonize they all undergo MET.
http://www.sciencedirect.com/science?_ob=ArticleListURL&_method=list&_ArticleListID=-862095745&_sort=r&_st=13&view=c&md5=66c53d8a325478606d9be13089d2aef8&searchtype=a
http://www.ncbi.nlm.nih.gov/pubmed/?term=epithelial+mesenchymal+transition+review
Obviously metastatic cells require MET after EMT for colonization.
My opinion is that cells injected directly into the circulation do not require EMT as the early stages of metastasis has been bypassed. There is no reqirement for dissociation of tumor cells from the primary site or invasion or intravasation. They will require to extravasate once they reach the secondary site. Most tumor cells injected iv will die those that survive will implant in the target parenchyma. it is believed that epithelial type cells are more efficient at doing this than mesenchymal like cells, hence the reqirement for MET when cells go through the full metastatic cascade. Iif the tumor cells injected are largely epithelial like anyway MET transformation would not be needed. Poorly differentiated colorectal cancer cells for example tend to be more invasive but less metastatic to the liver when injected intra splenically than the more epithelial like well differentiated cells.
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