Snehaa Chandrakumar

13 Questions 78 Answers 0 Followers

Questions related from Snehaa Chandrakumar

Difficult to figure out the concentration range for customizes oligos?

Dear Researchers, I have purchased my designed oligos from Sigma for miR based silencing studies as sense (~64nts) and antisense (~64nts); together if annealed it is ~64bp. I take equimolar...

01 January 2018 342 5 View

Difficult to figure out the concentration range for customizes oligos?

Dear Researchers, I have purchased my designed oligos from Sigma for miR based silencing studies as sense (~64nts) and antisense (~64nts); together if annealed it is ~64bp. I take equimolar...

01 January 2018 5,410 7 View

How to overcome the issues in cloning of amiRNA(with miR155 backbone into mammalian vector(pcDNA3.1-eSIBR(intronic region containing BIC region)-GFP)?

Dear Researchers, I have custom designed mammalian vector pcDNA3.1 containing the eSIBR region that has the BIC sequence and GFP downstream to the CMV promoter. The eSIBR region has a polylinker...

11 November 2017 9,706 0 View

Dear Researchers, Could anyone suggest me to overcome the troubles in cloning siRNA with an miRNA backbone into mammalian vector?

A siRNA is replaced in the sense and anti sense regions of an Mirna without disturbing it's backbone and is placed in a synthetic intronic region. I have the intronic region with a poly linker...

10 October 2017 9,704 0 View

Why and how DNA degrades after elution?

Hello Researchers, I am trying to clone my target genes into a promoter region of a vector (pcDNA3.1-GFP). I use 1 ug/ml of vector for restriction (20 ul volume) and I follow Invitrogen purelink...

04 April 2017 2,011 6 View

Can miRs from different chromosomal loci interact with each other?

Dear Researchers, Its well known that miRs after their complete biogenesis are released into the cytoplasm and they involve in different functions. I do have read from literature that certain miR...

03 March 2016 6,731 6 View

What are the possibilities of fungal contamination apart from handling?

MCF-7 cells were already handled perfectly and were good. Currently, we are meeting fungal contamination within the next passage. There are no much possibilities of handling errors as we do have...

09 September 2015 2,889 12 View

Do the plant extracts influence the staining intensity?

I use plant extracts of varying concentrations from 1 ng to 10 ug for estimating lipids in 9 day old 3T3-L1 adipocytes by Oil Red O staining. The cells are treated with the extracts throughout the...

07 July 2015 5,113 8 View

Does copper sulphate influence the growth of 3T3-L1 cells incubated in CO2 incubator?

3T3-L1 cells grow healthily when the humidity of the incubator is maintained by using a tray of water but it grows unhealthily and slow when the copper sulphate solution is used to maintain...

02 February 2015 8,979 4 View

Is it acceptable to get a percentage value higher than 100 for my glucose uptake assay in 3T3-L1 cells?

3T3-L1 cells are insulin sensitive but when they are counted and seeded for insulin stimulated glucose uptake assay, it results with 123% of glucose being up-taken by the positive control cells...

01 January 2015 920 7 View

Why do diabetic type 2 patients feel pain (disesthesias) in their legs?

The common symptoms of diabetic type 2 patients are numbness, disesthesias, vision blur. What are the mechanisms behind these? Can it be related to obesity?

04 April 2014 7,711 2 View

Can anyone help me out on review articles regarding damnacanthal and scopoletin?

Regarding its anti cancerous property. Is it possible to bring out the production of that in a bacteria?

01 January 2014 9,497 4 View

Could anyone suggest the reasons for visibility of showing low intense bands seen in PCR control (without template) compared to Test templates in PCR?

While running on 2% AGE (with product size ~320 bp), the negative control (Plasmid without insert) and the PCR control (without template) show bands with less intensity where there are no bands in...

01 January 1970 1,729 4 View