Sansrity Sinha

8 Questions 77 Answers 0 Followers

Questions related from Sansrity Sinha

How to determine if mean evolutionary rate (MER)computed by MEGA is statistically significant?

I used MEGA5.0 for computing MER for my dataset. the rates were computed based on JTT with an account of invariant sites model using 2 gamma categories for all positions in the alignment. these...

08 August 2018 5,458 1 View

Is it possible to remove just one node from a phylogenetic tree without actually making the tree again?

From the tree that i have I wanted to remove one of the nodes. is it possible to remove that node from the tree file?

01 January 2017 3,938 7 View

Is it possible to express and purify proteins in large quantities from mammalian cell lines ?

generally bacteria is used for protein expression in purification whenever protein is required in large quantities for structural studies. Is it possible to get such large amounts of proteins from...

08 August 2016 6,582 7 View

Can one replace one cloned shRNA with the other from pSUPER retro puro vector by merely removing it by using modified restriction cloning sites?

I have a pSUPER retor puro vector that already has a cloned shRNA. I wanted to remove the already cloned shRNA and replace it with the other shRNA. the user manual from oligoengine sys that upon...

07 July 2016 4,149 4 View

What is the general cut off used in CD-HIT when using a data set of closely related sequences for phyognetic analyses?

my dataset consists of highly similar sequences of proteins. i only wanted to remove duplicate sequeces if any in the data set. what threshold shall I set in CD HIT?

04 April 2016 5,538 1 View

How long does one need to incubate samples for digestion with dpn1?

I could amplify plasmid with primers i designed fkr making sdm n now i want to set ul dpn1 digestion. How long should be the incubation period?

11 November 2015 6,470 4 View

How does one select the best fit model for constructing phylogenetic trees?

I am working on mitofusins which are of two types in humans (not isofoms, but two different genes encoding them). i want to know at what time in evolution did the duplication happen. also which...

06 June 2015 5,045 6 View

How can I optimize antibiotic concentrations for generating a gene of interest expressing stable cell line?

I have my gene of interest cloned in mammalian expression system. there are two gene that i am looking for. i have cloned each of them in two different plasmids both having different promotors of...

06 June 2015 1,153 3 View