I want to know if ILC3s specifically, and more broadly all ILCs can be reconstituted by adoptive transfer of bone marrow cells from a widl-type mouse to a lethally irradiated knockout mouse.
Hi Mahima
In my hands they do, although the reconstitution is not super efficient. If you use a host that did have ILCs (WT or RagKO animals) you will have a lot of radio resistant host cells, and a low proportion of donor cells. Moreover, while RORg+ ILC3s are generated from donor origin, keep in mind that some ILC3s were suggested to develop in the embrionic life, so the proportion of ILC3 subpopulations might be altered.
Hi Mahima,
Yes, ILC3 should be reconstituted after BM Tx into lethally irradiated mice, though, as Diogo mentioned, the populations might be different than naive animals. In my hands, I see preferential reconstitution of Lti cells (Lin- Rorgt+ NKp46-) in mice reconstituted with WT BM and a lot of radio resistant host cells of this phenotype remaining. However, reconstitution of NKp46+ c-kit lo ILC3 as well as NKp46- c-kit- ILC3 is similar to naive mice. I transfer 5*10^6 total BM cells after radiation. Good luck.
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