Adam M. Farkas

5 Questions 12 Answers 0 Followers

Questions related from Adam M. Farkas

Monocytes not coming off plastic after culture w/ adsorbed antibody?

Hi, After coating a 48-well plate with a monocyte-specific antibody, then adding monocytes, it is impossible to harvest the cells (i.e. with vigorous pipetting, trypsin, or EDTA). If there are...

01 November 2024 8,311 2 View

How to align serial tissue sections in Image J?

Hi, I have IHC staining on 2-3 serial FFPE tissue sections. I did my best to take pictures of the same regions on a brightfield microscope but I'd like to be able to align each section into a...

07 February 2018 3,600 5 View

Any tips on improving performance of FlowJo v10 on Mac OS X Sierra?

Hi All, I'm aware that FlowJo v10 requires Java 6 which is no longer supported by Mac OS X Sierra. I'm having issues with long hang times (stalling, spinning pinwheels, fans running on high) when...

10 November 2016 9,835 4 View

Are there any methods for speeding up Aquamount drying?

Hello All, Does anyone have tips for speeding up the drying time for tissue sections mounted with Aquamount? Generally I let the slides dry at RT overnight but wondered if there was a faster way....

11 June 2015 6,710 3 View

Does anyone have a good protocol for staining bacterial cells with a primary Ab followed by a fluorescently-conjugated secondary Ab?

Hi All, Does anyone have a good protocol for staining bacterial cells (in suspension culture) with a primary Ab followed by a fluorescently-conjugated secondary Ab? I'm just wondering primarily...

10 March 2015 589 2 View