Can I use data from a soil analysis in another publication if I am using a sample from the same coordinates?

Hello. My team is planning a project using soil from a desert that has been chemically analized already. the publication includes the data from this soil and the coordinates where it was sampled....

01 February 2020 1,275 5 View

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Good evening, I wanted to ask you about something I have been observing. I work in a laboratory that does cell biology and molecular biology and we usually have some reagents and kits in the...

11 April 2019 1,229 3 View

No title

Good evening, I wanted to ask you about something I have been observing. I work in a laboratory that does cell biology and molecular biology and we usually have some reagents and kits in the...

03 April 2019 7,966 3 View

Can I use a newer NanoDrop software for old models?

Good afternoon, In the laboratory where I work we have an old NanoDrop (ND1000) but it is a bit restrictive to use it because it does not work with Windows10, we only have one computer with...

02 March 2019 6,339 2 View

Does anyone know about the effect of the city's climate on cell culture?

The laboratory where I work is located in Barranco (Lima, Peru) where there is a lot of humidity (~90%) and our cultures get easily contaminated with molds and yeasts eventhough we are very...

02 March 2018 7,643 3 View

How to stop getting Aspergillus in cell culture?

Good morning, I am working in a laboratory where we do cell culture, but lately we have been having problems with aspergillus contamination. We have tryed many times cleaning the incubator with...

10 November 2017 5,437 3 View

Anyone knows or has information about Carolina's pGREEN plasmid?

Good morning. I am trying to obtain information about the sequence or at least a restriction map of Carolina's pGREEN plasmid. I am supposed to cut it with BbsI, but when I try to make a virtual...

09 October 2017 1,483 2 View

How many cells can I obtain from a 75 cm^2 Corning flask?

Hello, I would like to know how many cells can be obtained from a 75 cm^2 Corning flask, I will grow MCF-7 cells and I need to obtain at least 20 ug of total RNA, the protocol I will use for RNA...

12 April 2016 2,515 3 View

Why do we measure 30 pollen grains per species?

Good Evening, I'm doing my thesis in palinology and I was told that we always measure 30 pollen grains per species, but I can't find the reason for this sample size anywhere. Can someone help me...

29 December 2014 9,717 13 View

Why do we measure 30 pollen grains per species?

Good Evening, I'm doing my thesis in palinology and I was told that we always measure 30 pollen grains per species, but I can't find the reason for this sample size anywhere. Can someone help me...

11 December 2014 3,189 16 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

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17 February 2021 4,217 3 View

How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View