How many cells can I obtain from a 75 cm^2 Corning flask?

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Michael James Milevskiy

Hi Adolfo,

I routinely grow MCF-7 cells in T75 flask and near confluence I will have over 1x10^6 cells. You might be able to get just over 10ug from that, but if you used two flasks or a T175 that should be enough. Hope that helps.

Adolfo Ubidia-Incio

Thank you Michael, it helps a lot. I think I will use two or three flasks as we have already bought them.

No problem, hope it works out.