How to view the electrostatic potential value of a Gaussian09W result?

Hi all I am able to view the electrostatic potential surface using Gaussview to see the calculating result from Gaussian09W of a small molecule. However, the strength is represented by color. I am...

19 January 2021 5,448 3 View

Is K-silicate gel a porous material?

I coated a stone surface with K-siilcate. Apparently, it does noe seal the surface since the water penetrae the stone easily and the absorption coefficient does not change too much. However, the...

05 October 2020 3,435 5 View

Are there any reasech on loteprednol etabonate microemulsion or nanoemulsion?

The API (i.e. loteprednol etabonate )has poor solubility in water but has excellent solubility in Transcutol P which serves as cosurfactant in my microemulsion formulation. But when I added water...

24 September 2020 417 3 View

What type of graph is suitable for describing one variable and two dependent variables?

08 June 2020 9,347 3 View

How to analysis FTIR data?

02 January 2020 6,175 3 View

How to deprive ATP in cancer cell?

I want to increase chromatin compaction of cancer cell and to see the function of interested gene in this process. Is there any method to make it?

19 November 2019 5,972 1 View

Why do some vertebrates 's teeth expose?

We know that some terrestrial vertebrates has exposed teeth,some of them are for sexual displaying,some are for a weapon or a tool,but most terrestrial vertebrates' teeth are cover by lips,the...

20 September 2019 1,183 4 View

What is the most suitable protocol to extract DNA from 70% ethanol fixed cell suspensions acquired from FACS?

I'm trying to extract DNA from 70% ethanol fixed cell suspensions. Firstly, The cells are fixed and stained for cell cycle analysis and collected after flow cytometry. the question is that the...

27 January 2019 9,767 3 View

Why the counterparts of virus A infectious clone swapped by counterparts of virus B cDNA fragments, virus A plasmid changed to low-copy?

does someone knows why the counterparts of virus A infectious clone swapped by same region of virus B cDNA fragments, virus A plasmid changed to low-copy?

14 January 2019 5,720 2 View

What is the most stable and accurate reference sample for conductive atomic force microscopy (CAFM)?

06 September 2017 1,995 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Do anyone here can help us in the field of molecular docking?

We have synthesized various metal complexes with different environment around the metals. Now to see the application of synthesized complexes we need this study also. we are also interested in...

01 March 2021 1,899 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

Universal bacterial primers targeting a mRNA?

Hi, I'm looking for universal primers targeting a bacterial mRNA to quatify bacterial load by qPCR in a complexe mixture (eukaryote and prokaryote RNA). Ribosomal RNA being depleted, I absolutly...

22 February 2021 9,969 3 View