A technology has been constructed in my lab whereby I can grasp a large DNA fragment of double kb from an E. coli genome, and then transfer it into another genome in your desirable locus. I am looking for collaborators to expand the study.
What sort od collaboration you are looking for! i am working on sperm DNA damage in humans. Any idea how we can explot these two together!
Let me know
Thanks
I am working on microbe genome engineering. I hope we can collaborate.
I would like to collaborate what can I do?
Dear colleagues, I want to search for a conference paper. However, it is hard to find it. Who knows how to find an old conference paper online? Thank you. Wolf, McCarthy, Heat Transfer to...
14 May 2024 3,904 6 View
Are there any stanards for identifying posterior cervicals and anterior dorsalsin dinosaurs, especially in theropods,could the position of the parapophysis and the presence of ventral keel be...
29 April 2024 7,257 1 View
For example, when selling the service products, female seller has greater influence than male seller on customers' purchase intention. However, I want to find out when selling a normal product,...
01 April 2024 2,164 2 View
I found two different products, and for each product, I found a female and a male to sell them in different ways. 1. The respondents needed to answer the same questionnaire twice after the four...
01 April 2024 959 2 View
Hi all, I'm summarizing the research on aerosol absorbance parameters measured at different times and locations. However, I don't know how to eliminate the difference in sampling time when...
19 March 2024 4,431 1 View
I'd like to do a test of human blood hemolysis and I heard that people donate a drop of their own blood to do the test. Is there anyone who can tell me how to do this?
31 January 2024 3,426 2 View
Different lithium ion batteries have varies T1 temperature in the ARC test. It is widely accepted that the T1 temperature is related to the SEI decomposition. But which component in the SEI of...
14 December 2023 1,456 1 View
A TF protein fused with GST tag was transfered into E. coli DE3 CCs for purification. But I always detect my protein in the SEDIMENTATION but SUPERNATANT. When I use PBS buffer to solute the...
11 August 2023 9,187 0 View
When I used 0.5g of air-dried soil and added 1ml of chloroform to fumigation it overnight in a fume hood, then extract with 0.5mol/L NaHCO3 within pH=8.5, there was no significant change in...
30 July 2023 6,097 5 View
If using an electric heating plate for digestion
28 June 2023 9,884 1 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
We assume this to be true. We also assume that the vacuum bomb is the latest version of explosives with an explosive power of a few to ten kg of TNT equivalent. It has the unique characteristic of...
04 August 2024 4,534 1 View
Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope...
02 August 2024 7,843 0 View
or we can collaborate
01 August 2024 6,705 2 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View