Nanobiotechnology arthritis treatment research?

01 February 2017 7,136 0 View

GC, GC-MS, GC-MS-MS course?

Hello everybody I would like to know if there is a really good online course about GC, GC-MS, GC-MS-MS, or one of the best books about instrumental chemistry. I would like to improve my knowledge...

31 December 2016 7,614 2 View

Which is the difference between potentiometric and electrometric method?

Hello everyone: I need some help, I got confused about those kind of methods: potentiometric and electrometric method, is it a different property measure? Please, send me some link (PDF or really...

05 June 2016 177 3 View

How may I quantify or determinate magnesium carbonate in soil?

03 April 2016 6,954 6 View

How can I eliminate interferences of some ions from carbonate and bicarbonate analysis?

Hello everyone I will do a validation of carbonates and bicarbonates analysis in soils (through titration analysis) but I have found that would be some interferences of some ions present in the...

03 April 2016 1,838 4 View

Is there some interference with synthesis of polymeric nanoparticles?

Hello everyone I've been working with PLA nanoparticles forma drug delivery, but I had had some troubles with the synthesis: I have no changed anything in my procedure, and I got differents...

01 February 2015 8,374 6 View

How can I improve the synthesis of polymeric nanoparticles?

I'm preparing polymeric nanoparticles using emulsification solvent evaporation method, but I haven't got the characteristic emulsion that indicates the nanoparticles formation.

10 November 2014 9,181 8 View

How can I eliminate baseline noise in HPLC?

09 October 2014 7,324 11 View

How may I do an optimization of experimental design?

Hello everybody I'm doing an experimental design (23 factorial experiment), but I need to optimize that experimental design, I haven't found the exact information to do that, maybe using...

06 July 2014 3,059 6 View

Emulsification diffusion method?

When I add water to make the diffusion step, I see two phases. Is that normal ?

09 October 2013 2,225 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View