DNA isolation methods - DNA yield is lesser in micro wave method then in salting out method and commercial kit method. Why?

Mayilvanan Chinnaiyan @Mayilvanan-Chinnaiyan

25 November 2017 0 8K Report

We are isolating DNA from wax embedded Squamous cell carcinoma (SCC) tissues. We take tissue sections and isolate the DNA from that. We following 3 methods for a comparison study. Those methods are Microwave method, Salting our method and by using QIAamp DNA Mini Kit from QIAGEN. My question is among the 3 methods we get lesser DNA yield in microwave method then compared to other two methods. So, what could be the reason for the lesser amount of DNA in microwave method.

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