I wish to induce nephrotoxicity in Wistar rats by using nicotine. I would like to know how much concentration of nicotine to be used for the induction to animal. It would be thankful If u could kindly help me for my research.
We are isolating DNA from wax embedded Squamous cell carcinoma (SCC) tissues. We take tissue sections and isolate the DNA from that. We following 3 methods for a comparison study. Those methods...
24 November 2017 8,482 0 View
While isolating DNA how do we make sure that we have isolated only the DNA at the end not RNA? At which step the DNA alone get separated and which buffer and composition is used for that... Thank You
24 October 2017 7,255 4 View
In flow analysis for rough pipe using Large Eddy Simulation, how the roughness height and roughness constant value can be incorporated. Is it possible?....
26 February 2017 5,486 5 View
Looking for the thermophysical properties of copper oxide for size greater than 50 nm in diameter.
28 January 2017 6,237 2 View
Using Nanoparticle as discrete phase and air as continuous phase, how to do modelling for calculating the flow and deposition rate on walls of pipe having 90 degree bend. Kindly share some...
19 January 2017 9,726 2 View
I would like to know the important genes involved in adipogenesis regulatory mechanism during high fat fed condition in Wistar rats or during type-2 diabetic condition...
20 May 2014 8,617 4 View
I have attached the block diagram of the control. I'm trying to implement the SPWM switching sequence and control the matrix converter(Three phase to single phase). The output of the Matrix...
01 January 1970 7,562 2 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
Dear All I would like to know the minimum number of animals (rats) to be studied for a pilot/preliminary research on intradermal drug delivery. Can I proceed with 1 animal per group for a pilot work?
02 August 2024 4,570 1 View
I am conducting my research regarding blood coagulation in patients with CKD. I observed a reduction in post hemodialysis aPTT and PT values compared to pre hemodialysis values. But most of...
30 July 2024 3,467 0 View
Dear all, I am working on particle deposition in human's & rat's respiratory airways using CFD and I am looking for the 3D CAD file for my simulations (STEP or IGES format). If somone has such...
29 July 2024 1,092 2 View
i compare between the antioxidant and oxidative stress level between negative control rats (saline) and CCL4 intoxicated rats. i found the level of GSH and TAC is more in toxic group than normal....
26 July 2024 1,887 0 View
Background: I am developing an RdRp assay for Dengue virus-3 and need to express and isolate the NS5 protein's RdRp domain (~101 kDa). My expression vector includes a GST tag followed by a 6x His...
13 July 2024 9,950 3 View
anti anaemic potentials if hibiscus sabdariffa
01 July 2024 8,429 0 View
Hello! I would like to study newly formed vessels after spinal cord injury in rats. However, I do not know what is the most suitable method to check diameter or number of vessels and which...
01 July 2024 9,744 0 View
I have been looking at papers for protein expression and trying to follow the process. I'm using TB medium to do the process, and the paper says to grow to an OD600 = 1.8 at 37 degrees, then let...
30 June 2024 6,502 5 View