Why are we measuring gel content of NBR latex? Does gel content related to the amount of cross-linked in a polymer?

Hi good day to everyone! I am actually studying the gel content of synthetic latex. Refer to ASTM 3616, gel content is measure of insoluble rubbers in the chosen solvent. So, I just would like to...

07 February 2021 605 4 View

How to determine the number of cells before suspending them in 1x binding buffer in Annexin V FITC assay?

I am performing Annexin V FITC assay for adherent cells. According to the protocol described, the treated cells were trypsinised and collected, followed by washing them twice with cold PBS. The...

18 July 2020 8,695 5 View

Appearance of crossing point signal even in water during qPCR. Help?

We have been running qPCR to detect Fusarium in wheat grains. Suddenly out of nowhere, we kept getting crossing point signal in the blank (PCR water). We have done the following: ran with only...

01 March 2020 1,408 3 View

What are the factors that affect the compatibility between filler and solvent in fabrication of mixed matrix/composite membrane?

I've heard of compatibility between polymer and solvent used to dissolve it based on the solubility parameter. However, little have I found upon solvent and filler interaction and compatibility....

18 August 2019 5,376 1 View

How to perform data analysis for ranking of familiarity of foods?

the most familiar food was assigned to 1 and continue rank among the list of the foods until the least familiar food. How to perform weighted ranking or any suitable method can be suggested for...

21 September 2018 6,557 3 View

Why do my western blots look so odd?

The three images attached here are for 3 different proteins probed at the same time. The first one is GAPDH. Does anyone have any idea why (1) Despite reasonable Bradford assay results, GAPDH...

14 January 2018 5,544 3 View

Choice of electrolyte for characterizing Cu2O/CuO coated on Cu mesh?

I have Cu2O/CuO coated on Cu mesh and would like to characterize its electrochemical properties using CV and Impedance analysis. I have tried using 0.5M and 1M of Na2SO4 (scan rate 10mV and 20mV),...

03 September 2017 4,244 4 View

Peak height in qPCR melt curve analysis?

31 October 2016 1,428 5 View

Why my cells (Flp-in CV-1) precipitate after adding in the antibiotics?

The culture looks fine during maintenance but after transfection, precipitation forms immediately after I adding the antibiotics (hygromycin b) for positive cells selection. So I tested the fresh...

18 August 2016 2,955 3 View

Where do i buy Sybr green dye?

27 June 2016 6,138 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View