Why are we measuring gel content of NBR latex? Does gel content related to the amount of cross-linked in a polymer?

Hi good day to everyone! I am actually studying the gel content of synthetic latex. Refer to ASTM 3616, gel content is measure of insoluble rubbers in the chosen solvent. So, I just would like to...

07 February 2021 605 4 View

How to determine the number of cells before suspending them in 1x binding buffer in Annexin V FITC assay?

I am performing Annexin V FITC assay for adherent cells. According to the protocol described, the treated cells were trypsinised and collected, followed by washing them twice with cold PBS. The...

18 July 2020 8,695 5 View

Appearance of crossing point signal even in water during qPCR. Help?

We have been running qPCR to detect Fusarium in wheat grains. Suddenly out of nowhere, we kept getting crossing point signal in the blank (PCR water). We have done the following: ran with only...

01 March 2020 1,408 3 View

What are the factors that affect the compatibility between filler and solvent in fabrication of mixed matrix/composite membrane?

I've heard of compatibility between polymer and solvent used to dissolve it based on the solubility parameter. However, little have I found upon solvent and filler interaction and compatibility....

18 August 2019 5,376 1 View

How to perform data analysis for ranking of familiarity of foods?

the most familiar food was assigned to 1 and continue rank among the list of the foods until the least familiar food. How to perform weighted ranking or any suitable method can be suggested for...

21 September 2018 6,557 3 View

Why do my western blots look so odd?

The three images attached here are for 3 different proteins probed at the same time. The first one is GAPDH. Does anyone have any idea why (1) Despite reasonable Bradford assay results, GAPDH...

14 January 2018 5,544 3 View

Choice of electrolyte for characterizing Cu2O/CuO coated on Cu mesh?

I have Cu2O/CuO coated on Cu mesh and would like to characterize its electrochemical properties using CV and Impedance analysis. I have tried using 0.5M and 1M of Na2SO4 (scan rate 10mV and 20mV),...

03 September 2017 4,244 4 View

Peak height in qPCR melt curve analysis?

31 October 2016 1,428 5 View

Why my cells (Flp-in CV-1) precipitate after adding in the antibiotics?

The culture looks fine during maintenance but after transfection, precipitation forms immediately after I adding the antibiotics (hygromycin b) for positive cells selection. So I tested the fresh...

18 August 2016 2,955 3 View

Where do i buy Sybr green dye?

27 June 2016 6,138 3 View

Have anyone checked before Cyclic voltammograms of intial ITO substrate and E-teck?

As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...

23 February 2021 3,175 3 View

Fictitious Boundary Meshing Method (FBM) in Commercial FEA software?

Is it possible to implement FBM in a commercial FEA software package, such as FEMLAB, COMSOL, or open source software like Moose?

22 February 2021 2,968 1 View

No title

Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...

08 February 2021 7,285 1 View

How can I remove salts from crude seaweed extract (except dialysis)?

Hi, I am interested to obtain phenolic fractions from crude seaweed extracts (using ethanol) and test for their antioxidant properties. Since most of the in-vitro antioxidant assays are...

26 January 2021 3,889 5 View

SDS-PAGE aggregation problem?

I ran SDS-PAGE with 14% lower gel, 4 % upper gel, having brushing, pre-running empty gel for 30 min. The protein samples had been centrifuged with 14,000g for 10 min, and then the supernatants...

20 January 2021 172 3 View

How to distinguish pure and adulterated honey using NMR spectroscopy?

What are the common adulterants in commercial honey? how to identify pure honey and adulterated honey with the help of NMR spectroscopy? Is there any other technique to check purity of honey?

20 January 2021 5,456 12 View

Can anyone suggest a commercial MAT kit to detect Toxoplasma gondii antibodies in animals?

18 January 2021 5,438 2 View

What percentage torque ripple exists in electrical motors used in electric vehicles ?

I am trying to understand the significance/percentage of torque ripples that exist in electrical motors (under operating conditions) that are used in commercial electric vehicles. If someone could...

15 January 2021 5,691 3 View

Any indexes for assessment of the potential health risks, associated with medical substances content in commercial fish species?

Are there any indexes ( like Hazard Index) to calculate the potential health risk associated with consuming food (commercial fish species) which bioaccumulates medical substances like...

12 January 2021 7,945 3 View

Does anyone know good publications/studies on the calculation of the heat demand of buildings?

Hello everyone, we need to calculate the heat demand of commercial and industrial buildings. Ideally the paper contains one or several formulas, which can be used to calculate the heat demand....

04 January 2021 9,366 4 View