Hello dear friends!
Well, In our Lab we have a little issue with the agarose gel electrophoresis. When we run a PCR product we saw few times a strange band, in fact sometimes we had the strange band in our ladder (strange thing because is the same ladder we usually use for more than 6 months by now) not just in the PCR product.
We're using the same SBx1 buffer, made with the same SBx20 buffer, same quantity of agarose, electrophoresis camera, power supply, BrEd, and some tests with GelRed and we have the same issue.
The funny thing is that just some times we have this issue, because we run at day like 3-5 pcr gels but just in some of them we have this issue.
Some suggestions about what its going on here?
Images: TrnlF1 7-03-15 P.cembroides var.bicolor.JPG image is the rare one, the 03.20.15-PruebaCajas.JPG is a sample because we think was a electrophoresis camera, so we perform a test, but in both cameras worked fine, and the 21feb2015 image is a GelRed test of a PCR product, but its just fine too.
Thank you for your time :D
Alex.
Hi there,
my 2 cents would be that you also might have a problem with that particular gel - the staining looks quite uneven suggesting that the EtBr isn't mixed in very well.
Good luck!
Dan
I was facing a similar issue while doing PCR with a good proof reading enzyme.
I found out that my Buffer as well as my water was contaminated. When changed to new sets of buffer and water I got good results.
Regards.!
Thank you Lawrence for the tip, yeah I was thinking that was the buffer, maybe is contaminated, I'll check it tomorrow!
Thank you all for your advices!
Take Care!
Alex.
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