Alejandro Gonzalez-Treviño

5 Questions 16 Answers 0 Followers

Questions related from Alejandro Gonzalez-Treviño

How can I remove Non-specific bands when I'm using degenerated COI primers?

Hello dear friends. I have done several PCRs using COI Variant primers (very long COI primers... 45pb Forward, 44pb Reverse), they have like 5-8 degenerated nucleotides that we made for specific...

28 March 2017 291 1 View

How can I run a dendrogram with weighted characters?

Hello dear researchers! Well, I have a little issue about how to run a dendrogram using a binary character matrix (1 = present 0 = absent) with the software MESQUITE 3.04. I already ran a UPGMA...

18 December 2015 10,089 3 View

DNA Agarose gel electrophoresis trouble, incomplete strange bands, any suggestions?

Hello dear friends! Well, In our Lab we have a little issue with the agarose gel electrophoresis. When we run a PCR product we saw few times a strange band, in fact sometimes we had the strange...

21 March 2015 3,439 3 View

How to decontaminate the working zone with Ethidium bromide

Hello dear friends. Well, we finally standardized GelRed dye and we want to decontaminate all the working area. We know that there is some protocols to follow to do this, but we don't know what...

16 May 2014 343 13 View

How expensive is it to work with SNPs?

I want to start a new project about swimming crab demographic evolution, and some specialists suggested some modern ways to get that information, and I read about SNPs and sound like a very good...

26 November 2013 2,451 4 View