Hi everyone,
I am trying to create a resistant cell line to a drug. Indeed, the first time i treated the cells with it , cells stopped growing then they grew again, when i passaged the cells to a smaller plate they started growing even faster..To my surprise, after treating the control cells (DMSO) with the same concentration that stopped the resistant line, they did not get effected by the drug and continued to grow.. could DMSO have an influence on the cells that made them so potent! or the small area of the plate could play a part! i am really confused at this point
i appreciate any advices on this matter . thank you
Very interesting observation. But i think you should repeat the experiment for further understanding. But as far as i have the experience that DMSO does not do much in resistance when used at less concentration. however drug resistance vary according to the seeding density. It may be possible that in small plate seeding density increased leading to better growth.
At low concentrations, DMSO is capable of inducing cell proliferation in some cancer cell lines such as human myeloma cells (~0.2-0.4% v/v). DMSO serves as a differentiating agent, e.g. In human preosteoblast cells (~1%).
Hello
Yes thhis due to low concentrations of DMSO which result to increase cell growth and proliferation
http://www.sciencedomain.org/abstract/5945
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