Hi everyone,
I used to see my protein of interest on my blot for all my cell lines but suddenly when I rerun the experiment I don't see it anymore in one cell line! even though I still use the same antibody and I see my protein on other cell lines. I suspected that there was something wrong with the lysate so I tried lysing new cells again and it gave the same result! no bands at all!
Any ideas or suggestions on how I can revive my protein? because I have the same expression for my loading control for all cell lines so I know that there is something there >_< it's driving me mad lol