Dear all, In the past I use a siimple and fast method to prepare my tissue or cell lysates. Usually snap freeze the tissue and after direct dissociation (sonication/ultrathurax) and boiling, protein determination and then WB.

Is there people that use directly Laemmli buffer, sonicate and heat denature before perfoming SDS gel and western blot?

Protocols are welcome!

Marc

More Marc Le Bert's questions See All
Similar questions and discussions