Nisha Sinha

5 Questions 7 Answers 0 Followers

Questions related from Nisha Sinha

How does spike-in adjust for variable inputs of RNA (when RNA is taken in the form of volume) for Real Time PCR in exosomes?

Hello Researchers, In most of the articles for RT-PCR of exosomes its suggested to take RNA input as volume (say 6 ul) with addition of spike-ins (for normalization of RT-PCR) and not amount due...

14 April 2022 445 3 View

Different speed for cells and urine derived exosomes?

Why is urinary exosomes isolated at 200000 g whereas at 120000 g for cell derived exosomes as mentioned in several papers. Will not these two different speeds yield different vesicles of...

03 April 2022 9,743 0 View

Can we store exosome in lysis buffer of miRVana RNA isolation kit so that total RNA from all the samples can be isolated the same day.

We are using ultracentrifugation for isolating exosomes.All the samples cannot be processed the same day.So can we store the exosomes in lysis buffer of miRVana miRNA isolation kit at -80 so that...

18 July 2017 7,943 3 View

Should the Ct value of the reference gene be less than the target gene in miRNA RT-PCR?

I have performed RT-PCR for exosomal miRNA. For normalisation I've used U6. But Ct value of the target gene is less than the normalised gene (U6). U6 is giving a Ct value of approx 32 whereas...

02 March 2015 8,649 5 View

Is it possible to generate non proteinuric diabetic cell culture model?

I would like to study diabetics with protein and diabetics without protein at molecular level in cell culture model. Is it possible to have non proteinuric diabetic in vitro model. Kindly suggest.

10 January 2015 626 1 View