Nisha Sinha

3 Questions 6 Answers 0 Followers

Questions related from Nisha Sinha

How does spike-in adjust for variable inputs of RNA (when RNA is taken in the form of volume) for Real Time PCR in exosomes?

Hello Researchers, In most of the articles for RT-PCR of exosomes its suggested to take RNA input as volume (say 6 ul) with addition of spike-ins (for normalization of RT-PCR) and not amount due...

14 April 2022 398 3 View

Different speed for cells and urine derived exosomes?

Why is urinary exosomes isolated at 200000 g whereas at 120000 g for cell derived exosomes as mentioned in several papers. Will not these two different speeds yield different vesicles of...

03 April 2022 9,673 0 View

Should the Ct value of the reference gene be less than the target gene in miRNA RT-PCR?

I have performed RT-PCR for exosomal miRNA. For normalisation I've used U6. But Ct value of the target gene is less than the normalised gene (U6). U6 is giving a Ct value of approx 32 whereas...

02 March 2015 8,607 5 View