I am planning to start with infection of my target cells with and I was wondering what would be the easiest method to quantify the virus titer so I could conclude about the optimal MOI? Any kind of protocol would be very much appreciated!
It is not the easiest, but I would recommend the TCID50 method, whereby a series of dilutions (I like to use 10 4-fold dilutions in quadruplicate) of your stock can be used to infect your target cells in a 96-well plate. Ideally, you would use the same target cells in your other assays to determine if a well has been infected. If this is not possible, the appropriate reporter cell line will have to be determined.