Matthew P Wood

6 Questions 10 Answers 0 Followers

Questions related from Matthew P Wood

Can antibody stripping formulations be used on live cells to recover surface epitopes prior to staining for flow cytometry?

I would like to measure the levels of specific cell surface proteins following treatment with an antibody drug. However, the drug itself occupies the same epitope (or a close enough epitope) to...

11 January 2022 9,871 0 View

Any murine antibodies targeting HIV SOSIP trimers? I would like to use a VRC01 or 447 type antibody in my imaging panel but would prefer a mouse mab?

I have several paraffin embedded draining lymph nodes from macaques vaccinated with HIV-1 SOSIP timers. I'd like to stain these tissues with a primary mouse anti-gp120 followed by a fluorescent...

23 March 2021 9,084 0 View

What is a good marker for imaging mouse macrophages by immunofluorescence in FFPE lymph nodes?

I am new to using mice, and I would like recommendations for specifically staining most, if not all macrophage subsets in murine tissues, particularly in lymph nodes and spleen. My background is...

17 November 2020 3,618 3 View

Can I image AlexaFluor488-labeled proteins in fixed tissue?

I'm planning to administer an immunogen labeled with AlexaFluor-488 into macaques with the goal to image the immunogen in the draining lymph node after it is biopsied. I'm having trouble finding...

22 September 2020 7,382 3 View

What are the kinetics of IFN-induced protein/cytokine expression in human monocytes?

I am designing an intracellular cytokine staining panel to assess TLR-induced type 1 IFN responses in human blood monocytes. I'm not sure if I should try staining for IFN directly, or some IFN...

14 April 2020 1,359 0 View

Any protocols for culturing BCG out of vaccinated animal tissue/cells?

Hello, I have some vials LN2 frozen immune cells from the draining lymph nodes of BCG-vaccinated macaques. I want to know if BCG is in these cells, and since I suspect it will be in very few of...

24 May 2018 5,335 2 View