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I am planning to start with infection of my target cells with and I was wondering what would be the easiest method to quantify the virus titer so I could conclude about the optimal MOI? Any kind...
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I am planning to test the efficiency of designed gRNA by performing a T7 assay, and I was wondering whether someone would have a protocol for Lipofectamine™ 2000 transfection of NIH-3T3 cells? I...
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After I have cloned my gRNAs into the lentiCRISPRv2 vector and sent the construct for sequencing, I have noticed that a single mismatch between my clone and original sequence has been introduced....
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